SEEDLING PRODUCTION OF Mimosa calodendron Mart. ex Benth. IN A TEMPORARY IMMERSION BIOREACTOR
نویسندگان
چکیده
ABSTRACT: Micropropagation is one technology to propagate endemic species of the Ferruginous Rupestrian Grasslands when in vitro genetic conservation sought. The present study aimed assess breaking dormancy, establishment, multiplication, elongation, rooting, and acclimatization Mimosa calodendron from culture a temporary immersion bioreactor system. seeds used for experiments were plants originating Grasslands. percentage contamination, oxidation, unresponsive seeds, germination, number buds per explant, shoot length, senescence, adventitious assessed. dormancy was most successful by mechanical scarification (80% germination). Immersion sodium hypochlorite 5 minutes efficient treatment establishment (90%). For multiplication elongation phase, use liquid medium cultivation suitable characteristics explant (2.55 buds), vigor (1.1), oxidation (1.3) senescence according score’s scale. Regardless method, percentages rooting satisfactory, it possible obtain complete 190 days. Palavras-chave: propagation; Grasslands; species. Produção de mudas Mart. ex Benth. em biorreator imersão temporária RESUMO: A micropropagação é uma alternativa para propagação espécies endêmicas do Campo Rupestre Ferruginoso, quando se busca conservação genética vitro. O trabalho teve como objetivo avaliar superação dormência, estabelecimento vitro, multiplicação, alongamento, enraizamento e aclimatização partir cultivo sistema (BIT). As sementes utilizadas os experimentos foram provenientes plantas oriundas Ferruginoso. porcentagem contaminação, oxidação, não responsivas, germinação, número gemas por explante, comprimento brotos, senescência, avaliados. dormência escarificação mecânica germinação) foi mais adequada dormência. hipoclorito sódio minutos o tratamento eficiente Para fase multiplicação alongamento uso meio líquido adequado as características explante (2,55 gemas), (1,1), oxidação (1,3) senescência acordo à escala notas. Independentemente método satisfatórios, sendo possível obter completas dias. Keywords: vitro; Ferruginoso; espécie endêmica.
منابع مشابه
Production of transgenic strawberries by temporary immersion bioreactor system and verification by TAIL-PCR
BACKGROUND Strawberry (Fragaria x ananassa) is an economically important soft fruit crop with polyploid genome which complicates the breeding of new cultivars. For certain traits, genetic engineering offers a potential alternative to traditional breeding. However, many strawberry varieties are quite recalcitrant for Agrobacterium-mediated transformation, and a method allowing easy handling of l...
متن کاملEvaluation of a New Temporary Immersion Bioreactor System for Micropropagation of Cultivars of Eucalyptus, Birch and Fir
The use of liquid instead of solid culture medium for the micropropagation of plants offers advantages such as better access to medium components and scalability through possible automation of the processes. The objective of this work was to compare a new temporary immersion bioreactor (TIB) to solid medium culture for the micropropagation of a selection of tree species micropropagated for comm...
متن کاملIn Vitro Propagation of Damask Rose Using the Temporary Immersion System
For centuries, Damask rose has been one of the most popular species of the rose family. Damask rose can be propagated using vegetative methods such as micropropagation. In this research, the use of temporary immersion system was reported for the first time for Damask rose micropropagation. For this reason, a traditional temporary immersion system was designed to facilitate its micropropagation....
متن کاملEffects of liquid, temporary immersion bioreactor and solid culture systems on micropropagation of Lilium ledebourii via bulblet microscales— An endangered valuable plant with ornamental potential
Lilium ledebourii (Baker) Boiss. (Liliaceae) is a critically endangered lily species native tonorthern Iran, where it is protected by law. In order to develop a cost effective method for largescalepropagation, the effects of three culture systems (solid, liquid and temporary immersion)and two types of cytokinins [6-Benzyladenine (BA) and Thidiazuron (TDZ)] were studied onthe in vitro plant rege...
متن کاملA New Triterpenoid from Terminalia glaucescens (Planch. ex Benth.)
Phytochemical investigation of the root extract of Terminalia glaucescens afforded three known compounds, ellagic acid 1, arjungenin 2, hypatic acid 3 and and a new triterpenoid named glaucescic acid, 2α,3α,6α,23-tetrahydroxyolean-12-en-28-oic acid 4. The structure and relative configuration of this new compound was elucidated on the basis of spectroscopic data, especially 2D NMR techniques.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nativa
سال: 2022
ISSN: ['2318-7670']
DOI: https://doi.org/10.31413/nativa.v10i1.13351